Fusobacterium nucleatum interacts with cancer-associated fibroblasts to promote colorectal cancer [* Cross-Reference *]

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Jessica Karta; Marianne Meyers; Fabien Rodriguez; Eric Koncina; Cedric Gilson; Eliane Klein; Monica Gabola; Mohaned Benzarti; Pau Pérez Escriva; José Alberto Molina‐Tijeras; Catarina Correia Tavares Bernardino; Falk Ponath; Anaïs Carpentier; Mònica Aguilera Pujabet; Maryse Schmoetten; Mina Tsenkova; Perla Saoud; Anthoula Gaigneaux; Dominik Ternes; Lidia Alonso; Nikolaus Zügel; Eric Willemssen; Philippe Koppes; Daniel Léonard; Luis Perez Casanova; Serge Haan; Michel Mittelbronn; Johannes Meiser; Vitaly I. Pozdeev; Jörg Vogel; Paolo Nucíforo; Paul Wilmes; Elisabeth Letellier, 2026, "Fusobacterium nucleatum interacts with cancer-associated fibroblasts to promote colorectal cancer [* Cross-Reference *]", https://lore.list.lu/citation?persistentId=perma:LIST.YWGAEX, LIST Open Repository, V1

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Description	Gut microbial species contribute to colorectal cancer (CRC) by interacting with tumor or immune cells, however if CRC-associated bacteria engage with stromal components of the tumor microenvironment remains unclear. Here, we report interaction between the CRC-associated bacterium Fusobacterium nucleatum and cancer-associated fibroblasts (CAFs), and show that F. nucleatum is present in the stromal compartment in murine CRC models in vivo and can attach to and invade CAFs. F. nucleatum-exposed CAFs exhibit a pronounced inflammatory-CAF (iCAF) phenotype, marked by elevated expression of established iCAF markers, secretion of pro-inflammatory cytokines such as CXCL1, IL-6 and IL-8, generation of reactive oxygen species (ROS), and an increased metabolic activity. In co-culture experiments, the interaction of cancer cells with F. nucleatum-stimulated CAFs enhances invasion, a finding further validated in vivo. Altogether, our results point to a role for the tumor microbiome in CRC progression by remodeling the tumor microenvironment through its influence on cancer-associated fibroblasts, suggesting novel therapeutic strategies for targeting CRC. (2025-08-22) *This entry has been automatically imported via OpenAlex by LIST harvest scripts. Please refer to https://doi.org/10.1038/s44318-025-00542-w for the original and latest version of the publication* (2026-07-01)
Subject	Chemistry; Medicine, Health and Life Sciences
Keyword	Fusobacterium nucleatum, Biology, Stromal cell, Tumor microenvironment, Colorectal cancer, Cancer research, Cancer-Associated Fibroblasts, Cancer, Cancer cell, Tumor progression, Immune system, Immunology, Bacteria
License/Data Use Agreement	CC BY 4.0


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Citation Metadata

Persistent Identifier	perma:LIST.YWGAEX
Publication Date	2026-07-06
Title	Fusobacterium nucleatum interacts with cancer-associated fibroblasts to promote colorectal cancer [* Cross-Reference *]
Other Identifier	https://doi.org/10.1038/s44318-025-00542-w OpenAlex ID: https://openalex.org/W4413435101
Author	Jessica Karta (University of Luxembourg, Luxembourg Institute of Science and Technology, Arbed (Luxembourg)) - ORCID: https://orcid.org/0000-0002-4674-0720 Marianne Meyers (University of Luxembourg, Luxembourg Institute of Science and Technology, Arbed (Luxembourg)) - ORCID: https://orcid.org/0000-0003-0475-1373 Fabien Rodriguez (University of Luxembourg, Luxembourg Institute of Science and Technology, Arbed (Luxembourg)) Eric Koncina (University of Luxembourg, Luxembourg Institute of Science and Technology, Arbed (Luxembourg)) - ORCID: https://orcid.org/0000-0003-4764-1510 Cedric Gilson (University of Luxembourg, Luxembourg Institute of Science and Technology, Arbed (Luxembourg)) - ORCID: https://orcid.org/0000-0001-7257-1097 Eliane Klein (University of Luxembourg, Luxembourg Institute of Science and Technology, Arbed (Luxembourg)) - ORCID: https://orcid.org/0000-0003-1433-9710 Monica Gabola (University of Luxembourg, Luxembourg Institute of Science and Technology, Arbed (Luxembourg)) - ORCID: https://orcid.org/0000-0002-5617-251X Mohaned Benzarti (University of Luxembourg, Luxembourg Institute of Science and Technology, Arbed (Luxembourg)) - ORCID: https://orcid.org/0000-0002-6413-3983 Pau Pérez Escriva (University of Luxembourg, Luxembourg Institute of Science and Technology, Arbed (Luxembourg)) - ORCID: https://orcid.org/0000-0002-2330-4248 José Alberto Molina‐Tijeras (University of Luxembourg, Luxembourg Institute of Science and Technology, Arbed (Luxembourg)) - ORCID: https://orcid.org/0000-0003-1554-9226 Catarina Correia Tavares Bernardino (University of Luxembourg, Luxembourg Institute of Science and Technology, Arbed (Luxembourg)) Falk Ponath (University of Würzburg) - ORCID: https://orcid.org/0000-0002-2144-0545 Anaïs Carpentier (Laboratoire National de Santé) - ORCID: https://orcid.org/0009-0001-6871-3301 Mònica Aguilera Pujabet (Vall d'Hebron Institute of Oncology) Maryse Schmoetten (University of Luxembourg, Luxembourg Institute of Science and Technology, Arbed (Luxembourg)) Mina Tsenkova (University of Luxembourg, Luxembourg Institute of Science and Technology, Arbed (Luxembourg)) - ORCID: https://orcid.org/0000-0001-9814-7921 Perla Saoud (University of Luxembourg, Luxembourg Institute of Science and Technology, Arbed (Luxembourg)) - ORCID: https://orcid.org/0000-0003-2611-3268 Anthoula Gaigneaux (University of Luxembourg, Luxembourg Institute of Science and Technology, Arbed (Luxembourg)) - ORCID: https://orcid.org/0000-0003-0465-3773 Dominik Ternes (University of Luxembourg, Luxembourg Institute of Science and Technology, Arbed (Luxembourg)) - ORCID: https://orcid.org/0000-0002-3507-6799 Lidia Alonso (Vall d'Hebron Institute of Oncology) Nikolaus Zügel (Centre Hospitalier de Luxembourg) Eric Willemssen (Luxembourg Institute of Health) Philippe Koppes (Luxembourg Institute of Health) Daniel Léonard (Luxembourg Institute of Health) - ORCID: https://orcid.org/0000-0002-9787-2133 Luis Perez Casanova (Laboratoire National de Santé) Serge Haan (University of Luxembourg, Luxembourg Institute of Science and Technology, Arbed (Luxembourg)) Michel Mittelbronn (University of Luxembourg, Luxembourg Institute of Science and Technology, Laboratoire National de Santé, Arbed (Luxembourg)) - ORCID: https://orcid.org/0000-0002-2998-052X Johannes Meiser (Luxembourg Institute of Health) - ORCID: https://orcid.org/0000-0002-9093-6210 Vitaly I. Pozdeev (University of Luxembourg, Luxembourg Institute of Science and Technology, Arbed (Luxembourg)) - ORCID: https://orcid.org/0000-0002-6526-9890 Jörg Vogel (University of Würzburg, Helmholtz Centre for Infection Research, Helmholtz Institute for RNA-based Infection Research) - ORCID: https://orcid.org/0000-0003-2220-1404 Paolo Nucíforo (Vall d'Hebron Institute of Oncology) - ORCID: https://orcid.org/0000-0003-1380-0990 Paul Wilmes (University of Luxembourg, Luxembourg Institute of Science and Technology) - ORCID: https://orcid.org/0000-0002-6478-2924 Elisabeth Letellier (University of Luxembourg, Luxembourg Institute of Science and Technology, Arbed (Luxembourg)) - ORCID: https://orcid.org/0000-0001-8242-9393
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Description	Gut microbial species contribute to colorectal cancer (CRC) by interacting with tumor or immune cells, however if CRC-associated bacteria engage with stromal components of the tumor microenvironment remains unclear. Here, we report interaction between the CRC-associated bacterium Fusobacterium nucleatum and cancer-associated fibroblasts (CAFs), and show that F. nucleatum is present in the stromal compartment in murine CRC models in vivo and can attach to and invade CAFs. F. nucleatum-exposed CAFs exhibit a pronounced inflammatory-CAF (iCAF) phenotype, marked by elevated expression of established iCAF markers, secretion of pro-inflammatory cytokines such as CXCL1, IL-6 and IL-8, generation of reactive oxygen species (ROS), and an increased metabolic activity. In co-culture experiments, the interaction of cancer cells with F. nucleatum-stimulated CAFs enhances invasion, a finding further validated in vivo. Altogether, our results point to a role for the tumor microbiome in CRC progression by remodeling the tumor microenvironment through its influence on cancer-associated fibroblasts, suggesting novel therapeutic strategies for targeting CRC. (2025-08-22) *This entry has been automatically imported via OpenAlex by LIST harvest scripts. Please refer to https://doi.org/10.1038/s44318-025-00542-w for the original and latest version of the publication* (2026-07-01)
Subject	Chemistry; Medicine, Health and Life Sciences
Keyword	Fusobacterium nucleatum Biology Stromal cell Tumor microenvironment Colorectal cancer Cancer research Cancer-Associated Fibroblasts Cancer Cancer cell Tumor progression Immune system Immunology Bacteria
Topic Classification	Gut microbiota and health Pancreatic and Hepatic Oncology Research Pharmacological Effects of Natural Compounds
Deposit Date	2025-08-22
Data Type	Article
Data Source	The EMBO Journal

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