Unveiling the global urban virome through wastewater metagenomics [* Cross-Reference *]

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Worp, Nathalie; Nieuwenhuijse, David F.; Izquierdo-Lara, Ray W.; Schapendonk, Claudia M.E.; Brinch, Christian; Jensen, Emilie Egholm Bruun; Munk, Patrick; Hendriksen, Rene S.; Larsson, D. G.Joakim; Berglund, Fanny; Woolhouse, Mark; Bunnik, Bram van; Kwenda, Geoffrey; Do Phuc, Nguyen; Tran, Doan Minh Nguyen; Van, Chinh Dang; Baklayan, Patricia; Rigamonti, Natalia; Wittum, Thomas E.; Kang, Sanghoon; Lilienthal, Ronald; Truong, Phuong; Sukapanpatharam, Pardi; Beck, Nicola Koren; Davis, Michael; Sobsey, Mark D.; Meschke, John Scott; Brown, Joe; Sozzi, Emanuele; Capone, Drew; Lefor, Brett; Gaze, William H.; Zhang, Lihong; Bunce, Joshua T.; Graham, David W.; Leonard, Anne; Moore, Andrew; Jassim, Laila Mohamed; Obaidi, Ouidiane; Thomsen, Jens; Kalule, John Bosco; Ejobi, Francis; Kurekci, Cemil; Levent, Belkis; Nikiema-Pessinaba, Somtinda Christelle; Njanpop-Lafourcade, Berthe Marie; Bidjada, Bawimodom; de Roda Husman, Ana Maria; Thamlikitkul, Visanu; Suthienkul, Orasa; Chonsin, Kaknokrat; Hounmanou, Yaovi Mahuton Gildas; Mdegela, Robinson Hammerthon; Kumburu, Happiness Houka; Hong, Yu Ping; der Heijden, Tanja Van; Boss, Renate; Örn, Stefan; Norrgren, Leif; Rutgersson, Carolin; Charlemagne, Kareem; Pathirage, Sujatha; Premasiri, H. I.; Bandara, N. K.B.K.R.G.W.; Perera, R. R.D.P.; Cerdà-Cuéllar, Marta; Lopez, Mariano Gomez; Quintela-Baluja, Marcos; Song, Min Joon; Esterhuyse, Kerneels; Keddy, Karen; Trkov, Marija; Truska, Peter; Pastuchová, Katarína; Gavačová, Dagmar; Wuertz, Stefan; Zuniga-Montanez, Rogelio; Tay, Moon Y.F.; Radosavljevic, Vladimir; Gassama, Amy; Perry, Meghan Rose; Hong, Pei Ying; Burduniuc, Olga; Cojocaru, Radu; Kochubovski, Mihail; Bosevska, Golubinka; Yoon, Sukhwan; Hur, Hor Gil; Shin, Hanseob; Di Yoong Wen, Doris; Álvaro, Pedro; Martins, Jose; Rocha, Jaqueline; Manaia, Celia; Wasyl, Dariusz; Yauce, Felipe Campos; Sibulo, Ruby; Catangcatang, Kris; Magtibay, Bonifacio B.; Velasquez, Jorge Echevarria, 2026, "Unveiling the global urban virome through wastewater metagenomics [* Cross-Reference *]", https://lore.list.lu/citation?persistentId=perma:LIST.QXPWSX, LIST Open Repository, V1
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Description
Understanding global viral dynamics is critical for public health. Traditional surveillance focuses on individual pathogens and symptomatic cases, which may miss asymptomatic infections or newly emerging viruses, delaying detection and response. Wastewater-based epidemiology has been used to track pathogens through targeted molecular assays, but its reliance on predefined targets limits detection of the full viral spectrum. Here, we analyse longitudinal wastewater samples from 62 cities across six continents (2017–2019) using metagenomics and capture-based sequencing with probes targeting viruses associated with gastrointestinal disease. We detect over 2500 viral species spanning 122 families, many with human, animal, or plant health relevance. The bacteriophage family Microviridae and plant virus family Virgaviridae dominate the metagenomic dataset, while Astroviridae and Picornaviridae prevail in the capture-based sequence dataset. Virus distributions are broadly similar across continents at the family and genus levels, yet distinct city-level fingerprints reveal geographical and temporal variation, enabling spatiotemporal surveillance of viruses such as astroviruses and enteroviruses. Global wastewater-based epidemiology enables early detection of emerging viruses, including Echovirus 30 in Europe and Tomato brown rugose fruit virus. These findings highlight the potential of wastewater sequencing for the early detection of emerging viruses and population-wide virome monitoring across diverse hosts. (2025-12-01)
***This entry has been automatically imported via Infodoc(ASO) CSV by LIST harvest scripts. Please refer to https://doi.org/10.1038/s41467-025-65208-x for the original and latest version of the dataset and data downloads*** (2026-06-09)
Subject Earth and Environmental Sciences
Keyword dynamics is critical, critical for public, Understanding global viral, global viral dynamics, viral dynamics
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