Elucidating tumour‐associated microglia/macrophage diversity along glioblastoma progression and under ACOD1 deficiency [* Cross-Reference *]

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Yolanda Pires‐Afonso; Arnaud Muller; Kamil Grzyb; Anaïs Oudin; Yahaya A Yabo; Carole Sousa; Andrea Scafidi; Aurélie Poli; Antonio Cosma; Rashi Halder; Djalil Coowar; Anna Golebiewska; Alexander Skupin; Simone P. Niclou; Alessandro Michelucci, 2022, "Elucidating tumour‐associated microglia/macrophage diversity along glioblastoma progression and under ACOD1 deficiency [* Cross-Reference *]", https://lore.list.lu/citation?persistentId=perma:LIST.N7NYTD, LIST Open Repository, V1

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Description	In glioblastoma (GBM), tumour-associated microglia/macrophages (TAMs) represent the major cell type of the stromal compartment and contribute to tumour immune escape mechanisms. Thus, targeting TAMs is emerging as a promising strategy for immunotherapy. However, TAM heterogeneity and metabolic adaptation along GBM progression represent critical features for the design of effective TAM-targeted therapies. Here, we comprehensively study the cellular and molecular changes of TAMs in the GL261 GBM mouse model, combining single-cell RNA-sequencing with flow cytometry and immunohistological analyses along GBM progression and in the absence of Acod1 (also known as Irg1), a key gene involved in the metabolic reprogramming of macrophages towards an anti-inflammatory phenotype. Similarly to patients, we identify distinct TAM profiles, mainly based on their ontogeny, that reiterate the idea that microglia- and macrophage-like cells show key transcriptional differences and dynamically adapt along GBM stages. Notably, we uncover decreased antigen-presenting cell features and immune reactivity in TAMs along tumour progression that are instead enhanced in Acod1-deficient mice. Overall, our results provide insight into TAM heterogeneity and highlight a novel role for Acod1 in TAM adaptation during GBM progression. (2022-07-15) *This entry has been automatically imported via OpenAlex by LIST harvest scripts. Please refer to https://doi.org/10.1002/1878-0261.13287 for the original and latest version of the publication* (2026-07-01)
Subject	Chemistry; Medicine, Health and Life Sciences
Keyword	Microglia, Mass cytometry, Biology, Stromal cell, Reprogramming, Immune system, Immunotherapy, Cancer research, Glioma, Macrophage, Phenotype, Flow cytometry, Tumor progression, Tumor microenvironment, Cell, Immunology, Inflammation, Cancer, Gene, In vitro, Genetics
License/Data Use Agreement	CC BY 4.0


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Citation Metadata

Persistent Identifier	perma:LIST.N7NYTD
Publication Date	2026-07-06
Title	Elucidating tumour‐associated microglia/macrophage diversity along glioblastoma progression and under ACOD1 deficiency [* Cross-Reference *]
Other Identifier	https://doi.org/10.1002/1878-0261.13287 OpenAlex ID: https://openalex.org/W4285593191
Author	(University of Luxembourg, Luxembourg Institute of Health, Luxembourg Institute of Science and Technology)https://orcid.org/0000-0002-5773-414X (Luxembourg Institute of Health)https://orcid.org/0000-0002-3841-4722 (University of Luxembourg)https://orcid.org/0000-0002-2416-9500 (Luxembourg Institute of Health)https://orcid.org/0000-0001-5552-9823 (University of Luxembourg, Luxembourg Institute of Health, Luxembourg Institute of Science and Technology)https://orcid.org/0000-0002-1128-6038 (Luxembourg Institute of Health)https://orcid.org/0000-0002-8707-8796 (University of Luxembourg, Luxembourg Institute of Health, Luxembourg Institute of Science and Technology)https://orcid.org/0000-0002-3089-2439 (Luxembourg Institute of Health)https://orcid.org/0000-0002-7986-0761 (Luxembourg Institute of Health)https://orcid.org/0000-0002-3686-8034 (University of Luxembourg)https://orcid.org/0000-0002-1402-1254 (University of Luxembourg)https://orcid.org/0000-0001-6330-0156 (Luxembourg Institute of Health)https://orcid.org/0000-0002-4160-2521 (University of Luxembourg, University of California San Diego)https://orcid.org/0000-0002-8955-8304 (Luxembourg Institute of Health, University of Bergen)https://orcid.org/0000-0002-3417-9534 (Luxembourg Institute of Health)https://orcid.org/0000-0003-1230-061X
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Description	In glioblastoma (GBM), tumour-associated microglia/macrophages (TAMs) represent the major cell type of the stromal compartment and contribute to tumour immune escape mechanisms. Thus, targeting TAMs is emerging as a promising strategy for immunotherapy. However, TAM heterogeneity and metabolic adaptation along GBM progression represent critical features for the design of effective TAM-targeted therapies. Here, we comprehensively study the cellular and molecular changes of TAMs in the GL261 GBM mouse model, combining single-cell RNA-sequencing with flow cytometry and immunohistological analyses along GBM progression and in the absence of Acod1 (also known as Irg1), a key gene involved in the metabolic reprogramming of macrophages towards an anti-inflammatory phenotype. Similarly to patients, we identify distinct TAM profiles, mainly based on their ontogeny, that reiterate the idea that microglia- and macrophage-like cells show key transcriptional differences and dynamically adapt along GBM stages. Notably, we uncover decreased antigen-presenting cell features and immune reactivity in TAMs along tumour progression that are instead enhanced in Acod1-deficient mice. Overall, our results provide insight into TAM heterogeneity and highlight a novel role for Acod1 in TAM adaptation during GBM progression. (2022-07-15) *This entry has been automatically imported via OpenAlex by LIST harvest scripts. Please refer to https://doi.org/10.1002/1878-0261.13287 for the original and latest version of the publication* (2026-07-01)
Subject	Chemistry; Medicine, Health and Life Sciences
Keyword	Microglia Mass cytometry Biology Stromal cell Reprogramming Immune system Immunotherapy Cancer research Glioma Macrophage Phenotype Flow cytometry Tumor progression Tumor microenvironment Cell Immunology Inflammation Cancer Gene In vitro Genetics
Topic Classification	Immune cells in cancer Neuroinflammation and Neurodegeneration Mechanisms Single-cell and spatial transcriptomics
Deposit Date	2022-07-15
Data Type	Article
Data Source	Molecular Oncology

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