immunoHuMiX: A personalizable gut‐on‐chip model for unraveling human microbiome–immune interactions [* Cross-Reference *]

Version 1.0
Charlotte De Rudder; Kacy Greenhalgh; Joanna Baginska; Camille Martin‐Gallausiaux; Léa Grandmougin; Rémy Villette; Mara Lucchetti; Bérénice Hansen; Marek Ostaszewski; Viacheslav A. Petrov; Koji Atarashi; Lena Weidert; Audrey Frachet Bour; Dominik Ternes; Javier Ramiro‐Garcia; Antoine Malabirade; Takeshi Tanoue; Marc Mac Giolla Eain; Elisabeth Letellier; Matthew Barrett; Jérôme Lacombe; Frederic Zenhausern; Coralie Guérin; Markus Ollert; Carole Seguin‐Devaux; Kenya Honda; Paul Wilmes, 2026, "immunoHuMiX: A personalizable gut‐on‐chip model for unraveling human microbiome–immune interactions [* Cross-Reference *]", https://lore.list.lu/citation?persistentId=perma:LIST.KGFR6I, LIST Open Repository, V1
Learn about Data Citation Standards.
Dataset Metrics
0 Downloads
Description
An individual's immune status is an essential factor affecting the efficacy of microbiome-based therapies. To enable representative studies of microbiome–immune system interactions, we developed protocols to facilitate the coculture of intestinal epithelial cells and human peripheral blood mononuclear cells (PBMCs) in our microphysiological model of the human gut, HuMiX. Using the resulting immunoHuMiX model, we assessed responses to human–microbial cocultures with a probiotic mixture comprised of 17 strictly anaerobic Clostridial strains (17-mix). We demonstrated the ability of our model to sustain metabolically active microbiota, while maintaining human cell viability, and generated relevant read-outs for host–microbiome interaction studies. The composition of the strain mixture was not affected by the individual sources of PBMCs, and the abundances of the different immune cell populations were comparable when incubated with or without the 17-mix after 24 h in the system, and between individuals. We observed increased cytokine production, PBMC viability, and PBMC cell counts upon coculture with the 17-mix, thereby demonstrating immune sensing of the microbiota by the PBMCs. In summary, our immunoHuMiX model provides an important personalizable tool for screening microbiota-driven immune responses. (2026-03-24)
***This entry has been automatically imported via OpenAlex by LIST harvest scripts. Please refer to https://doi.org/10.1002/viw.20250213 for the original and latest version of the publication*** (2026-07-01)
Subject Chemistry; Engineering; Medicine, Health and Life Sciences; Physics
Keyword Immune system, Peripheral blood mononuclear cell, Cytokine, Probiotic, Cell, Human cell, Strain (injury), Cell type
License/Data Use Agreement

CC BY 4.0

There are no files in this dataset.
Edit File

This file has already been deleted (or replaced) in the current version. It may not be edited.

Restrict Access

Restricting limits access to published files. People who want to use the restricted files can request access by default. If you disable request access, you must add information about access to the Terms of Access field.

Learn about restricting files and dataset access in the User Guide.

Enable access request
You must enable request access or add terms of access to restrict file access.
Save Changes
Edit Embargo

The selected file or files have already been published. Contact an administrator to change the embargo date or reason of the file or files.
Edit Retention Period

The selected file or files have already been published. Contact an administrator to change the retention period date or reason of the file or files.
Delete Files

The file will be deleted after you click on the Delete button.

Files will not be removed from previously published versions of the dataset.

Select File(s)

Please select one or more files.

Share Dataset

Share this dataset on your favorite social media networks.

Continue
Dataset Citations

Citations for this dataset are retrieved from Crossref via DataCite using Make Data Count standards. For more information about dataset metrics, please refer to the User Guide.

Sorry, no citations were found.
Inaccessible Files Selected

The selected file(s) may not be downloaded because you have not been granted access or the file(s) have a retention period that has expired or the files can only be transferred via Globus.

You may request access to any restricted file(s) by clicking the Request Access button.

Ineligible Files Selected

The selected file(s) may not be transferred because you have not been granted access or the file(s) have a retention period that has expired or the files are not Globus accessible.

You may request access to any restricted file(s) by clicking the Request Access button.

Download Options

The files selected are too large to download as a ZIP.

You can select individual files that are below the 100.0 MB download limit from the files table, or use the Data Access API for programmatic access to the files.

Select File(s)

Please select a file or files to be downloaded.

Inaccessible Files Selected

The selected file(s) may not be downloaded because you have not been granted access or the file(s) have a retention period that has expired.

Click Continue to download the files you have access to download.

Ineligible Files Selected

Some file(s) cannot be transferred. (They are restricted, embargoed, with an expired retention period, or not Globus accessible.)

Click Continue to transfer the elligible files.

Delete Dataset

Are you sure you want to delete this dataset and all of its files? You cannot undelete this dataset.

Delete Draft Version

Are you sure you want to delete this draft version? Files will be reverted to the most recently published version. You cannot undelete this draft.

Unpublished Dataset Preview URL

Preview URL can only be used with unpublished versions of datasets.

Unpublished Dataset Preview URL

Are you sure you want to disable the Preview URL? If you have shared the Preview URL with others they will no longer be able to use it to access your unpublished dataset.

Delete Files

The file(s) will be deleted after you click on the Delete button.

Files will not be removed from previously published versions of the dataset.

Compute

This dataset contains restricted files you may not compute on because you have not been granted access.

Deaccession Dataset

Are you sure you want to deaccession? This is permanent and the selected version(s) will no longer be viewable by the public.

Deaccession Dataset

Are you sure you want to deaccession this dataset? This is permanent, and it will no longer be viewable by the public.

Version Differences Details

Please select two versions to view the differences.

Version Differences Details
 
Version:
Last Updated:
Version:
Last Updated:
Select File(s)

Please select a file or files for access request.

Select File(s)

Embargoed files cannot be accessed. Please select an unembargoed file or files for your access request.

Edit Tags

Select existing file tags or create new tags to describe your files. Each file can have more than one tag.

Request Access

 You need to Log In to request access.

Dataset Terms

Please confirm and/or complete the information needed below in order to request access to files in this dataset.

This dataset is made available under the following terms. Please confirm and/or complete the information needed below in order to continue.

Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation shown on the dataset page.

CC BY 4.0

Preview Guestbook

Upon downloading files the guestbook asks for the following information.

Account Information

Package File Download

Use the Download URL in a Wget command or a download manager to download this package file. Download via web browser is not recommended. User Guide - Downloading a Dataverse Package via URL

https://lore.list.lu/api/access/datafile/

Compute Batch
Clear Batch
Dataset Persistent Identifier Change Compute Batch
Compute Batch
Submit for Review

You will not be able to make changes to this dataset while it is in review.

Publish Dataset

Are you sure you want to republish this dataset?

Select if this is a minor or major version update.

Publish Dataset

This dataset cannot be published until LIST Open Repository is published by its administrator.

Return to Author

Return this dataset to contributor for modification. The reason for return entered below will be sent by email to the author.

Curation Status History
StatusDateAssigner
No records found.
Add/Edit a Version Note
Styled Citation