Circulating immune profiling reveals impaired monocyte states and trajectories driving immunosuppression in glioblastoma [* Cross-Reference *]

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Andrea Scafidi; Tony Kaoma; Claudia Cerella; Eleonora Campus; Kamil Grzyb; Bakhtiyor Nosirov; Frida Lind‐Holm Mogensen; Eliane Klein; Raul Da Costa; Alexander Skupin; Frank Hertel; Guy Berchem; Michel Mittelbronn; Antonio Cosma; B. Melin; Simone Niclou; Petr V Nazarov; A Golebiewska; Aurélie Poli; Alessandro Michelucci, 2026, "Circulating immune profiling reveals impaired monocyte states and trajectories driving immunosuppression in glioblastoma [* Cross-Reference *]", https://lore.list.lu/citation?persistentId=perma:LIST.HMXXUV, LIST Open Repository, V1

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Description	Abstract Glioblastoma (GBM) is an aggressive and lethal brain tumor marked by profound local and systemic immune dysfunction. Yet, the diagnostic and therapeutic relevance of peripheral impairments remains undefined. To clinically dissect their underlying mechanisms and pathological implications, we combined mass and flow cytometry with single-cell RNA-sequencing of peripheral blood mononuclear cells from GBM patients and healthy donors. GBM blood profiles were characterized by heterogeneous changes in classical monocytes, encompassing expanded, reduced and unchanged subsets, presenting distinct functional states, including antigen-presenting, interferon and metabolic subsets. Additional adaptations included myeloid-derived suppressor cell (MDSC) expansion and loss of non-classical monocytes. Trajectory analyses positioned MDSCs as an intermediate state, in continuum with the metabolic subset. Single-cell RNA-sequencing further showed antigen-presenting monocyte propensity to differentiate into tumor-associated macrophages. Circulating monocytes shared a “GBM-classical monocytic signature” exhibiting low MHC class II expression, altered cell-cell communication and increased anti-inflammatory mediators, such as IL1R2 and CD163 . Lastly, lymphocyte alterations included decreased proportions of CD4 + T, natural killer (NK) and CD56 + T cells, retaining relatively conserved activation profiles, exemplified by up-regulation of alarmins S100A8/S100A9 . These findings map systemic immune reprogramming in GBM, suggesting new avenues for non-invasive biomarker discovery and therapeutic strategies to restore anti-tumor immunity. (2026-01-06) *This entry has been automatically imported via OpenAlex by LIST harvest scripts. Please refer to https://doi.org/10.64898/2026.01.06.697857 for the original and latest version of the publication* (2026-07-01)
Subject	Chemistry; Medicine, Health and Life Sciences
Keyword	Mass cytometry, Immune system, Monocyte, Immunosuppression, Peripheral blood mononuclear cell, Flow cytometry, Acquired immune system, Immunotherapy, Reprogramming, Suppressor
License/Data Use Agreement	CC BY 4.0


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Persistent Identifier	perma:LIST.HMXXUV
Publication Date	2026-07-06
Title	Circulating immune profiling reveals impaired monocyte states and trajectories driving immunosuppression in glioblastoma [* Cross-Reference *]
Other Identifier	https://doi.org/10.64898/2026.01.06.697857 OpenAlex ID: https://openalex.org/W7118728041
Author	Andrea Scafidi (University of Luxembourg, Luxembourg Institute of Health, Luxembourg Institute of Science and Technology) - ORCID: https://orcid.org/0000-0002-3089-2439 Tony Kaoma (Luxembourg Institute of Health) - ORCID: https://orcid.org/0000-0002-1269-4826 Claudia Cerella (Luxembourg Institute of Health) - ORCID: https://orcid.org/0000-0001-9308-8176 Eleonora Campus (University of Luxembourg, Luxembourg Institute of Health, Luxembourg Institute of Science and Technology) Kamil Grzyb (University of Luxembourg) - ORCID: https://orcid.org/0000-0002-2416-9500 Bakhtiyor Nosirov (Luxembourg Institute of Health) - ORCID: https://orcid.org/0000-0002-4644-2758 Frida Lind‐Holm Mogensen (University of Luxembourg, Luxembourg Institute of Health, Luxembourg Institute of Science and Technology) - ORCID: https://orcid.org/0000-0002-3497-2321 Eliane Klein (Luxembourg Institute of Health) - ORCID: https://orcid.org/0000-0003-1433-9710 Raul Da Costa (Luxembourg Institute of Health) - ORCID: https://orcid.org/0000-0001-9666-8301 Alexander Skupin (University of Luxembourg, University of California San Diego) - ORCID: https://orcid.org/0000-0002-8955-8304 Frank Hertel (Centre Hospitalier de Luxembourg) Guy Berchem (Centre Hospitalier de Luxembourg) - ORCID: https://orcid.org/0000-0003-0157-2257 Michel Mittelbronn (University of Luxembourg, Luxembourg Institute of Health, Luxembourg Institute of Science and Technology, Laboratoire National de Santé) - ORCID: https://orcid.org/0000-0002-2998-052X Antonio Cosma (Luxembourg Institute of Health) - ORCID: https://orcid.org/0000-0002-3686-8034 B. Melin (Umeå University) Simone Niclou (University of Luxembourg, Luxembourg Institute of Health, Luxembourg Institute of Science and Technology) Petr V Nazarov (Luxembourg Institute of Health) - ORCID: https://orcid.org/0000-0002-4568-7382 A Golebiewska (Luxembourg Institute of Health) Aurélie Poli (Luxembourg Institute of Health) - ORCID: https://orcid.org/0000-0002-7986-0761 Alessandro Michelucci (Luxembourg Institute of Health) - ORCID: https://orcid.org/0000-0003-1230-061X
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Description	Abstract Glioblastoma (GBM) is an aggressive and lethal brain tumor marked by profound local and systemic immune dysfunction. Yet, the diagnostic and therapeutic relevance of peripheral impairments remains undefined. To clinically dissect their underlying mechanisms and pathological implications, we combined mass and flow cytometry with single-cell RNA-sequencing of peripheral blood mononuclear cells from GBM patients and healthy donors. GBM blood profiles were characterized by heterogeneous changes in classical monocytes, encompassing expanded, reduced and unchanged subsets, presenting distinct functional states, including antigen-presenting, interferon and metabolic subsets. Additional adaptations included myeloid-derived suppressor cell (MDSC) expansion and loss of non-classical monocytes. Trajectory analyses positioned MDSCs as an intermediate state, in continuum with the metabolic subset. Single-cell RNA-sequencing further showed antigen-presenting monocyte propensity to differentiate into tumor-associated macrophages. Circulating monocytes shared a “GBM-classical monocytic signature” exhibiting low MHC class II expression, altered cell-cell communication and increased anti-inflammatory mediators, such as IL1R2 and CD163 . Lastly, lymphocyte alterations included decreased proportions of CD4 + T, natural killer (NK) and CD56 + T cells, retaining relatively conserved activation profiles, exemplified by up-regulation of alarmins S100A8/S100A9 . These findings map systemic immune reprogramming in GBM, suggesting new avenues for non-invasive biomarker discovery and therapeutic strategies to restore anti-tumor immunity. (2026-01-06) *This entry has been automatically imported via OpenAlex by LIST harvest scripts. Please refer to https://doi.org/10.64898/2026.01.06.697857 for the original and latest version of the publication* (2026-07-01)
Subject	Chemistry; Medicine, Health and Life Sciences
Keyword	Mass cytometry Immune system Monocyte Immunosuppression Peripheral blood mononuclear cell Flow cytometry Acquired immune system Immunotherapy Reprogramming Suppressor
Topic Classification	Immune cells in cancer Glioma Diagnosis and Treatment Single-cell and spatial transcriptomics
Deposit Date	2026-01-06
Data Type	Preprint
Data Source	bioRxiv (Cold Spring Harbor Laboratory)

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