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Persistent Identifier
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perma:LIST.DHZRNA |
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Publication Date
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2026-07-06 |
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Title
| A High-Quality Phased Genome Assembly of Stinging Nettle (Urtica dioica ssp. dioica) [* Cross-Reference *] |
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Other Identifier
| https://doi.org/10.3390/plants14010124
SCOPUS_ID:85214497091 |
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Author
| Hirabayashi, Kaede (Michael Smith Laboratories)Michael Smith LaboratoriesORCID0000-0001-6557-8098
Dumigan, Christopher R. (University of British Columbia Okanagan)University of British Columbia OkanaganORCID0000-0002-3326-8375
Kučka, Matúš (University of British Columbia Okanagan, Slovak University of Agriculture in Nitra)University of British Columbia Okanagan, Slovak University of Agriculture in NitraORCID0000-0003-1219-3519
Percy, Diana M. (The University of British Columbia, The University of British Columbia)The University of British Columbia, The University of British ColumbiaORCID0000-0002-0468-2892
Guerriero, Gea (Luxembourg Institute of Science and Technology)Luxembourg Institute of Science and TechnologyORCID0000-0001-6586-6997
Cronk, Quentin (The University of British Columbia, The University of British Columbia, The University of British Columbia)The University of British Columbia, The University of British Columbia, The University of British ColumbiaORCID0000-0002-4027-7368
Deyholos, Michael K. (University of British Columbia Okanagan)University of British Columbia OkanaganORCID0000-0003-4205-3891
Todesco, Marco (Michael Smith Laboratories, University of British Columbia Okanagan, The University of British Columbia, The University of British Columbia)Michael Smith Laboratories, University of British Columbia Okanagan, The University of British Columbia, The University of British ColumbiaORCID0000-0002-6227-4096 |
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Point of Contact
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LIST RDS (LIST) |
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Description
| Stinging nettles (Urtica dioica) have a long history of association with human civilization, having been used as a source of textile fibers, food and medicine. Here, we present a chromosome-level, phased genome assembly for a diploid female clone of Urtica dioica from Romania. Using a combination of PacBio HiFi, Oxford Nanopore, and Illumina sequencing, as well as Hi-C long-range interaction data (using a novel Hi-C protocol presented here), we assembled two haplotypes of 574.9 Mbp (contig N50 = 10.9 Mbp, scaffold N50 = 44.0 Mbp) and 521.2 Mbp (contig N50 = 13.5 Mbp, scaffold N50 = 48.0 Mbp), with assembly BUSCO scores of 92.6% and 92.2%. We annotated 20,333 and 20,140 genes for each haplotype, covering over 90% of the complete BUSCO genes and including two copies of a gene putatively encoding the neurotoxic peptide urthionin, which could contribute to nettle’s characteristic sting. Despite its relatively small size, the nettle genome displays very high levels of repetitiveness, with transposable elements comprising more than 60% of the genome, as well as considerable structural variation. This genome assembly represents an important resource for the nettle community and will enable the investigation of the genetic basis of the many interesting characteristics of this species. (2025-01-01)
***This entry has been automatically imported via Infodoc(ASO) CSV by LIST harvest scripts. Please refer to https://doi.org/10.3390/plants14010124 for the original and latest version of the dataset and data downloads*** (2026-06-09) |
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Subject
| Earth and Environmental Sciences |
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Keyword
| chromosomal structural variation
fiber crops
genome assembly
Hi-C protocol
stinging nettle
trichomes
Urtica dioica |
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Funding Information
| Alliance de recherche numérique du Canada: RGPIN-2023-03344 |
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Deposit Date
| 2025-01-01 |
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Data Type
| Article |
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Data Source
| Plants; eISSN: 22237747, vol. 14, n° 1, 2025 |
