Application of a real-time reverse transcription polymerase chain reaction for rapid detection of Escherichia coli in drinking water: an EU representative study [* Cross-Reference *]

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Livia Gómez; João Brandão; Anna Navarro; Antonino Martines; Isabella Sanseverino; Elena Porcel-Rodríguez; Dimitar Marinov; Oksana Ableitner; Franz Allerberger; Adrie Atsma; Rossella Briancesco; Arijana Cenov; Marianna Cíchová; Anna Maria Coccia; S. Della Libera; Katrien De Maeyer; Hendrik J. de Vries; Goffe Elsinga; Leo Heijnen; Anna-Maria Hokajärvi; Veronika Janská; Jerko Hrabar; Monika Huse-Kutowska; Slaven Jozić; Stefanie Klatovsky; Christian Kornschober; Katharina Kohls; Adrie Krom; Katrin Luden; Claudia Mikula-Pratschke; Tarja Pitkänen; Mateja Poje; Miloslava Prokšová; Matjaž Retelj; Beata Rozwadowska; Annastiina Rytkönen; Anniina Sarekoski; Sabine Schlager; Burkhard Springer; Iva Stojan; Eline Stroobach; Neven Sučić; Elisabete Valério; Gabi W. van Pelt; Jerom Vranken; Ana Vrdoljak Tomaš; Darija Vukić Lušić; Ewa Włos; Gerhard Wubbels; Maria Ziombska; Katja Zelenik; Marcin Ziuziakowski; Teresa Lettieri, 2025, "Application of a real-time reverse transcription polymerase chain reaction for rapid detection of Escherichia coli in drinking water: an EU representative study [* Cross-Reference *]", https://lore.list.lu/citation?persistentId=perma:LIST.BGZVHF, LIST Open Repository, V1
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The microbiological quality of water intended for human consumption in the EU is regulated by the recast Drinking Water Directive 2020/2184 (DWD), which sets strict parametric values for intestinal enterococci and Escherichia coli (E. coli), allowing no more than zero colony-forming units per 100 mL. Detection and enumeration of E. coli typically rely on culture-based reference methods or the most probable number approach, which require 1–2 days to produce results—potentially delaying timely action during contamination events. In contrast, molecular techniques can deliver results within hours. The DWD permits the use of alternative methods if they are as reliable as the reference method and developing and validating such methods requires multiple laboratory trials in line with ISO standard 16140-2. Following this, we conducted a representative EU study to validate a molecular method based on real-time reverse transcription polymerase chain reaction for rapid E. coli detection in drinking water. In a concerted action, the first of its kind for drinking water, nineteen laboratories across ten Member States participated. To ensure consistency, drinking water was artificially contaminated with E. coli. The alternative method showed slightly lower sensitivity than the reference method (91.1 % vs. 97.2 %) but delivered much faster results, making it a valuable screening tool. It can support rapid decision-making during contamination events, reducing the risk of waterborne outbreaks and protecting public health. For reliable routine performance, appropriate training in this alternative method is strongly recommended. (2025-05-10)
***This entry has been automatically imported via OpenAlex by LIST harvest scripts. Please refer to https://doi.org/10.1016/j.envres.2025.121786 for the original and latest version of the publication*** (2026-07-01)
Subject Chemistry; Earth and Environmental Sciences; Medicine, Health and Life Sciences; Physics
Keyword Escherichia coli, Polymerase chain reaction, Reverse transcription polymerase chain reaction, Reverse transcriptase, Chemistry, Real-time polymerase chain reaction, Biology, Microbiology, Molecular biology, Biochemistry, Gene, Gene expression
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